Human E3 ubiquitin-protein ligase CBL-B (CBLB) ELISA Kit

This Sandwich ELISA Kit from Assay Genie is designed for the precise measurement of CBLB (E3 ubiquitin-protein ligase CBL-B) levels in human samples. The kit is intended for research use only and supports studies in immunology, including investigation of CBLB's role as a regulator of the ubiquitin-proteasome system, with relevance to cancer, autoimmune disorders, and inflammatory conditions.

The kit is reactive with human samples and accepts serum, plasma, tissue homogenates, cell culture supernates and other biological fluids. Specificity is for natural and recombinant human E3 ubiquitin-protein ligase CBL-B. The target protein (UniProt: Q13191) is located in the cytoplasm and associates with endocytic vesicles upon EGF stimulation, with a research area focus on immunology.

Performance characteristics include a detection range of 78-5000pg/mL, sensitivity of 25pg/mL, intra-assay CV of 5.1%, and inter-assay CV of 8.6%. Linearity for serum (N=5) spans 97-107% at 1:2, 89-99% at 1:4, 91-100% at 1:8, and 109-118% at 1:16, with EDTA plasma at 105-115%, 100-112%, 116-125%, and 90-99% across the same dilutions, and heparin plasma at 103-115%, 82-91%, 99-109%, and 104-114%. Recovery is reported as 103% (97-109%) for serum and 105% (99-111%) for plasma.