Human E2F4 ELISA Kit (HUFI04507)

Human E2F4 ELISA Kit from Assay Genie enables quantitative measurement of E2F transcription factor 4, a ~44 kDa nuclear protein belonging to the E2F family of cell cycle regulators that acts predominantly as a transcriptional repressor during cellular quiescence.

The kit utilizes a sandwich immunoassay format on a pre-coated microplate and is configured for colorimetric readout on a standard microplate reader. It is validated for the specific detection of human E2F4 with no significant cross-reactivity reported with closely related E2F family members, and is supplied for research use only.

Applications include investigations into G1/S cell cycle transition control, tumor suppressor pathway analysis (particularly studies of the RB/E2F axis), and quantitative comparison of E2F4 abundance across sample cohorts. Specimens such as serum and plasma may be processed following standard laboratory protocols; samples should be aliquoted and stored under appropriate conditions to maintain analyte stability before measurement.