Human E-Selectin/CD62E SuperSet ELISA Kit

Designed for the quantitative measurement of human soluble E-Selectin/CD62E, this DIY ELISA kit from Assay Genie uses a colorimetric detection method. It is intended for use with serum, plasma, and cell culture supernatant samples, with a recommended sample volume of 100 µl per well.

The assay has a stated range of "1 ng/ml - 32 ng/ml" and a sensitivity of "0.5 ng/ml", and is specific for human soluble ELAM-1, recognising both natural and recombinant forms. No cross-reactivity with other human adhesion molecules is reported. Total incubation from sample to end is 2h15. Supplied components include capture and biotinylated detection antibodies, one standard per plate, Streptavidin-HRP, TMB substrate, and a detailed procedure including buffer composition. The kit is aimed at immunology research in pharmaceutical and biotech industries, and each kit contains a highly optimised antibody pair and a purified recombinant protein standard for detecting analyte in cell culture supernatants, serum, and plasma.