Human Dynein, Axonemal, Heavy Chain 11 (DNAH11) ELISA Kit

The DNAH11 protein is an axonemal dynein heavy chain that drives microtubule sliding in motile cilia and flagella. Assay Genie's ELISA kit enables quantitative measurement of this target in research samples, supporting studies of primary ciliary dyskinesia (PCD), respiratory epithelium function, and sperm motility, where loss-of-function variants in DNAH11 are linked to situs inversus, chronic sinusitis, bronchiectasis, and male infertility.

The microplate format provides parallel wells for standards and unknowns, with a standard curve generated from serially diluted recombinant antigen. Capture and detection antibodies are paired against human DNAH11 epitopes for quantification in tissue homogenates and cell lysates. Researchers run the assay at room temperature (18-25 degrees C), add 100 ul of diluted sample per well according to the supplied protocol, and read the chromogenic signal on a standard plate reader after stopping the reaction.

For storage, unopened kits should remain refrigerated; reagents remain stable through the printed expiry when handled as directed. All components are intended for research use only and are not validated for diagnostic procedures.