Human Dual specificity protein phosphatase 5 (DUSP5) ELISA Kit

From Assay Genie, this Sandwich ELISA kit is designed for the precise quantitative measurement of Human Dual specificity protein phosphatase 5 (DUSP5) in human serum, plasma, and cell culture supernatants. The kit uses an ELISA detection method and is intended for research use only. It shows specificity toward natural and recombinant human Dual specificity protein phosphatase 5.

Reported performance characteristics include a detection range of 0.156-10ng/mL and a sensitivity of 0.11ng/mL. Intra-assay CV is 7.2% and inter-assay CV is 10.5%. Linearity has been evaluated in serum, EDTA plasma, and heparin plasma across dilutions from 1:2 to 1:16. Average recovery is 98% for serum (range 92-104%) and 100% for plasma (range 94-106%). DUSP5 is a dual specificity protein phosphatase active toward phosphotyrosine, phosphoserine, and phosphothreonine residues, with the highest relative activity toward ERK1, and is localized to the nucleus. The UniProt accession is Q16690 and the reported molecular weight is 42kDa.

Validated sample types include serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids. The kit includes a dismountable ELISA microplate, lyophilized standard, sample diluent, assay diluents A and B, detection reagents A and B, wash buffer, substrate, stop solution, and plate sealers.