Human Dual Specificity Phosphatase 5 (DUSP5) ELISA Kit

This quantitative immunoassay from Assay Genie measures human Dual Specificity Phosphatase 5 (DUSP5) protein in research samples using a pre-coated microplate. DUSP5 is an inducible nuclear phosphatase that selectively inactivates ERK1 and ERK2 MAP kinases, making the kit useful to researchers studying cytokine signalling, oncogenic Ras pathway activity, and cellular stress responses.

The procedure follows a standard capture-and-detection workflow: standards and unknown samples are loaded into the wells, a biotinylated detection antibody recognises bound DUSP5, and a streptavidin-enzyme conjugate generates a chromogenic signal proportional to target abundance. Absorbance is then read on a standard microplate reader. Each kit is calibrated specifically for the human DUSP5 sequence and is supplied for research use only.

The kit supports up to 96 determinations and is validated for use on tissue homogenates. DUSP5 dysregulation has been documented in colorectal, breast, and lung cancer tissues, so investigators frequently pair this assay with gene expression analysis to confirm translational changes at the protein level. The protocol can be completed within a standard working day and requires only basic plate-washing and reading equipment.