Human Dual specificity mitogen-activated protein kinase kinase 5 (MAP2K5) ELISA Kit

A Sandwich ELISA Kit from Assay Genie, designed for the quantitative detection of human Dual specificity mitogen-activated protein kinase kinase 5 (MAP2K5). The assay is reactive with human samples, including serum, plasma, tissue homogenates, cell culture supernates and other biological fluids. It is presented as a reliable and sensitive tool for accurate detection of MAP2K5 levels, providing precise and reproducible results for a range of research applications.

Reported performance characteristics include a detection range of 0.312-20ng/mL and a sensitivity of 0.11ng/mL, with an intra-assay CV of 4.5% and an inter-assay CV of 7.5%. Across serial dilutions from 1:2 to 1:16, linearity for serum (N=5) spans 86-116%, EDTA plasma (N=5) 98-112%, and heparin plasma (N=5) 83-120%. Recovery averages 100% (range 94-106%) for serum and 102% (range 96-108%) for plasma. The kit specifically detects natural and recombinant human MAP2K5 (UniProt Q13163), also known by the synonyms MAPK/ERK kinase 5, MEK 5, MAP kinase kinase 5, MEK5, MKK5, and PRKMK5.

MAP2K5 is a dual specificity protein kinase that acts as a scaffold for the formation of a ternary MAP3K2/MAP3K3-MAP3K5-MAPK7 signaling complex; activation of this pathway has been linked to protection from stress-induced apoptosis, neuronal survival, and cardiac development and angiogenesis. Dysregulation of MAP2K5 has been associated with cancer, inflammatory disorders, and neurological conditions, making it a biomarker of research interest. The product is designated for research use only.