Human DPPA4 (Developmental pluripotency-associated protein 4) ELISA Kit (HUFI04549)

The DPPA4 protein (Developmental Pluripotency-Associated Protein 4) is a nuclear factor expressed in undifferentiated human embryonic stem cells and induced pluripotent stem cells, where it contributes to chromatin accessibility and maintenance of the pluripotent state. This Assay Genie kit provides quantitative measurement of the human isoform with capture antibodies raised specifically against human DPPA4, demonstrating no significant cross-reactivity with mouse or rat homologs. Each kit includes a pre-coated microplate, lyophilized reference standards, biotinylated detection antibody, streptavidin-HRP conjugate, substrate solution, and wash buffer sufficient for the full plate run. Reconstituted standards should be aliquoted and stored frozen, with working dilutions prepared fresh before each assay to limit freeze-thaw degradation. Intended for research use only, the kit supports investigations in germ cell tumor biology, somatic cell reprogramming efficiency, and characterization of pluripotent cell lines. Researchers profiling stem cell differentiation pathways or cancer stem cell populations can pair this ELISA with companion pluripotency markers such as OCT4, NANOG, and SSEA4 to map cellular identity transitions across reprogramming time courses.