Human Dopamine Transporter (DAT) ELISA Kit

Quantify the dopamine transporter (DAT) in human tissue homogenates and cell lysates with this ELISA Kit from Assay Genie. DAT, encoded by SLC6A3, is the presynaptic membrane protein that reuptakes dopamine from the synaptic cleft and is central to research on Parkinson's disease, ADHD, substance use disorders, and reward circuitry.

The assay accepts tissue homogenates and cell lysates, making it useful for brain-region dissections, striatal and midbrain preparations, and cultured dopaminergic cell models such as SH-SY5Y or differentiated LUHMES. Typical workflows include comparing DAT expression in healthy versus diseased tissue, monitoring knockdown efficiency in siRNA or CRISPR-edited lines, and screening test articles for activity at the transporter.

Each kit ships with a pre-coated microplate and the reagents needed to run the full protocol. Refrigerate components between uses and protect from light where the protocol indicates. Read the absorbance endpoint on a standard plate reader. Researchers in neuropharmacology, addiction biology, and neurodegeneration programs rely on this kit for dopamine-transporter studies in human samples.