Human Dopamine Beta Hydroxylase (DbH) ELISA Kit

DBH, the copper-dependent monooxygenase that catalyzes the conversion of dopamine to norepinephrine within chromaffin granules of the adrenal medulla and postganglionic sympathetic neurons, can be quantified with this immunoassay from Assay Genie. As a key enzyme in the catecholamine biosynthetic pathway, dopamine beta-hydroxylase is widely investigated as a biomarker for sympathetic nervous system activity, cardiovascular regulation, and stress-related physiology. Researchers apply this in vitro assay in studies of orthostatic hypotension, neurodegenerative disease models, psychiatric conditions such as schizophrenia and depression, and pheochromocytoma differential diagnostics. The kit is configured for research use only and includes a pre-coated microplate, biotinylated detection antibody, streptavidin-HRP conjugate, calibrated standards, wash buffer, substrate, and stop solution. The standard curve spans 0.156-10 ng/mL with a 100 µL sample volume per well, supporting quantification of human DBH in serum, plasma, and tissue homogenate samples. Store unused strips in the sealed pouch at 4 degrees C; reconstituted standards should be aliquoted and frozen at -20 degrees C to limit repeated freeze-thaw cycles. Each lot delivers reproducible standard curves across the working range, with intra-assay precision supporting reliable quantification across multiple sample matrices prepared under standardized collection and storage protocols.