Human Dopa Decarboxylase PharmaGenie ELISA Kit (SBRS0490)

The Human Dopa Decarboxylase PharmaGenie ELISA Kit from Assay Genie enables quantitative measurement of DDC, also known as aromatic L-amino acid decarboxylase (AADC) — the pyridoxal-phosphate-dependent enzyme that catalyzes the final decarboxylation step in monoamine biosynthesis, converting L-DOPA to dopamine and 5-HTP to serotonin. The antibody pair is human-specific, with no significant cross-reactivity with rodent DDC isoforms, supporting research on Parkinson's disease models, neuroblastoma cell lines, and neurotransmitter pathway analysis via ELISA. Validated for cell culture supernatants, plasma, and serum, the colorimetric immunoassay ships with all reagents required for a full microplate run of standards and unknowns. A typical sample volume is 100 uL per well, and the developed chromogen is quantified on a standard plate reader. Unopened kit components should be stored refrigerated; reconstituted standards should be aliquoted and frozen to limit freeze-thaw cycles, and chromogenic substrates are best protected from light.