Human Dnmt3b PharmaGenie ELISA Kit (SBRS0506)

DNA methyltransferase 3B (Dnmt3b) is a de novo methyltransferase that establishes CpG methylation patterns during mammalian development, and the Human Dnmt3b PharmaGenie ELISA Kit from Assay Genie enables quantitative measurement of this enzyme in research samples. Dnmt3b is required for genomic imprinting, X-chromosome inactivation, and silencing of endogenous retrotransposons. Biallelic loss-of-function mutations in DNMT3B cause ICF syndrome, while elevated Dnmt3b expression has been reported across multiple solid tumor types.

This immunoassay accepts cell culture supernatants, plasma, and serum specimens, enabling both in vitro cell-based studies and clinical research workflows. The kit ships with reagents for 96 individual determinations, supporting batch processing of experimental cohorts focused on epigenetic regulation.

Application areas include oncology biomarker research, stem cell differentiation studies, and pharmacological screening programs evaluating Dnmt3b inhibition as a therapeutic strategy. The microplate protocol runs with standard laboratory equipment and produces an absorbance-based readout compatible with conventional plate readers.

Storage temperature and shelf-life specifications appear on the enclosed product insert. Researchers should review the full protocol before initiating the assay. Each box contains all components required for the complete protocol run.