Human DNAJB3 ELISA Kit

A sandwich ELISA from Assay Genie, the Human DNAJB3 ELISA Kit uses a double antibody format with colorimetric detection for the quantitative measurement of DNAJB3 in human samples. Synonyms for the target include DNAJB3 and HCG3 (Uniprot Q8WWF6).

DNAJB3 is described as a key protein involved in cellular stress response and protein folding, with dysregulation linked to neurodegenerative disorders and cancer. The kit is intended for research in cell biology, and storage conditions are 2-8°C for 12 months.

Assay performance data: linearity at dilutions 1:2, 1:4, 1:8 (n=5) was 85-102%, 83-101%, 81-96% for serum; 87-99%, 82-100%, 81-94% for EDTA plasma; and 87-102%, 90-100%, 85-100% for heparin plasma. Recovery ranges with averages were serum 88-104% (94%), EDTA plasma 86-95% (91%), and heparin plasma 85-98% (93%). The protocol involves primary incubation at 37°C for 90 minutes, detection antibody binding for 60 minutes, HRP-streptavidin binding for 30 minutes, TMB substrate development for 10-20 minutes in the dark, and absorbance measurement at 450 nm.