Human DnaJ homolog subfamily B member 3 (DNAJB3) ELISA Kit

From Assay Genie, this sandwich ELISA kit enables quantitative detection of human DnaJ homolog subfamily B member 3 (DNAJB3), also known by the synonym HCG3. It is intended for research use only and is reactive with human samples, including serum, plasma, tissue homogenates, cell culture supernates and other biological fluids, with specificity for both natural and recombinant human DNAJB3.

Manufacturer-reported performance characteristics include a detection range of 0.156-10 ng/mL and a sensitivity of 0.056 ng/mL. Precision values are 5.9% intra-assay CV and 7.4% inter-assay CV. Spike-and-recovery testing gave an average of 100% (range 94-106%) in serum and 102% (range 96-108%) in plasma, and linearity was evaluated in serum, EDTA plasma and heparin plasma across dilutions of 1:2, 1:4, 1:8 and 1:16.

DNAJB3 is a member of the DNAJ (Hsp40) family of chaperone proteins and is annotated as a potential co-chaperone of male germ cell- and haploid stage-specific Hsp70 proteins. The human gene maps to chromosomal location 2q37, with UniProt accession Q8WWF6 and a reported molecular weight of 16,559 Da. Source commentary notes that abnormalities in DNAJB3 expression have been linked to neurodegenerative disorders and myopathies, making the protein of interest as a biomarker in related research.