Human DnaJ homolog subfamily B member 1 (DNAJB1) ELISA Kit

Assay Genie offers a sandwich ELISA kit designed for the accurate detection of human DnaJ homolog subfamily B member 1 (DNAJB1) in serum, plasma, tissue homogenates, cell culture supernatants and other biological fluids. The assay is intended for research use only and is suited to studies exploring the role of DNAJB1 in protein folding, cellular homeostasis and disease mechanisms.

The kit provides a detection range of 93.75-6000pg/mL with a sensitivity of 45pg/mL. Intra-assay CV is 3.7% and inter-assay CV is 6.9%. Specificity is directed against natural and recombinant human DnaJ homolog subfamily B member 1. Recovery has been validated in serum (average 88%, range 82-94%) and plasma (average 90%, range 84-96%), supporting reliable quantification across common clinical sample types.

DNAJB1 is a molecular chaperone that interacts with HSP70 and stimulates its ATPase activity, promoting protein folding and preventing misfolded protein aggregation. Dysregulation of DNAJB1 has been linked to cancer, neurodegenerative disorders and metabolic diseases, making it a valuable biomarker for investigating disease pathogenesis and potential therapeutic targets. The protein localises to the cytoplasm and translocates rapidly to the nucleus and especially to the nucleoli upon heat shock.