Human DNA replication licensing factor MCM2 (MCM2) ELISA Kit

Designed for the quantitative measurement of human DNA replication licensing factor MCM2 in serum, plasma, tissue homogenates, cell culture supernates and other biological fluids, this Sandwich ELISA Kit from Assay Genie provides a high-specificity tool for research applications exploring DNA replication and genome stability. The kit detects natural and recombinant human MCM2, a core component of the MCM2-7 helicase complex required for 'once per cell cycle' DNA replication initiation and elongation, S-phase entry, and cell division, and is classified under the Epigenetics research area.

Performance characteristics include a detection range of 0.078-5 ng/mL, a sensitivity of 0.039 ng/mL, an intra-assay CV of 5.3%, and an inter-assay CV of 9.4%. Linearity has been validated across serial dilutions of serum, EDTA plasma, and heparin plasma samples, with recovery averaging 97% (range 91-103%) in serum and 99% (range 93-105%) in plasma. The target protein is localised to the nucleus (UniProt P49736), and the kit is intended for research use only.