Human Dkk-1 ELISA Kit

Manufactured by Assay Genie, this Sandwich ELISA kit is designed for the quantitative measurement of Dkk-1 in human samples. The assay uses a double-antibody sandwich format with colorimetric detection, offering a sensitivity of 18.75 pg/mL and a standard range of 31.25–2000 pg/mL. Known synonyms for the target include DKK1, Dickkopf-1, and dickkopf-related protein 1.

The kit is validated for use with serum, plasma, cell culture supernatant, cell or tissue lysates, and other liquid samples, suiting diverse research workflows. DKK-1 is a key regulator of the Wnt signaling pathway, and abnormal levels have been associated with cancer, osteoporosis, and Alzheimer's disease, making it a valuable biomarker for investigating disease mechanisms and therapeutic targets.

The procedure involves reagent equilibration, primary incubation at 37°C for 90 minutes, biotin-labeled detection antibody incubation for 60 minutes, HRP-streptavidin binding for 30 minutes, and TMB substrate development in the dark for 10–20 minutes, followed by absorbance reading at 450 nm. Kit components include a pre-coated ELISA microplate, lyophilized standard, biotin-labeled antibody concentrate, HRP-streptavidin conjugate (SABC), TMB substrate, sample and antibody dilution buffers, SABC dilution buffer, stop solution, 25X wash buffer, plate sealers, and a technical manual. Components are stored at 2–8°C, with a shelf life of 12 months.