Human Disintegrin and metalloproteinase domain-containing protein 10 (ADAM10) ELISA Kit

Designed for the quantitative measurement of ADAM10 in human samples, this sandwich ELISA kit from Assay Genie targets the human Disintegrin and metalloproteinase domain-containing protein 10. ADAM10 is a key enzyme involved in cell adhesion, migration, and proteolysis, and its dysregulation has been implicated in cancer, Alzheimer's disease, and inflammation, making it relevant to studies of disease pathology and therapeutic development.

The assay is reactive with human samples and accepts serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids. It is specific for natural and recombinant human ADAM10. Reported performance characteristics include a detection range of 0.156–10 ng/mL, a sensitivity of 0.045 ng/mL, an intra-assay CV of 5.9%, and an inter-assay CV of 8.8%. Linearity has been validated across dilutions from 1:2 to 1:16 in serum (94–104% to 105–114%), EDTA plasma (110–120% to 105–114%), and heparin plasma (110–120% to 99–111%). Recovery averages 99% in serum (range 93–105%) and 101% in plasma (range 95–107%).

Synonyms for the target include CDw156, Kuzbanian protein homolog, Mammalian disintegrin-metalloprotease, CD156c, ADAM 10, KUZ, and MADM. The associated UniProt accession is O14672, and the kit is designated for research use only.