Human Discs, Large Homolog Associated Protein 5 (DLGAP5) ELISA Kit

DLGAP5, also called HURP or DLG7, is a microtubule-associated protein required for proper kinetochore-microtubule attachment and mitotic spindle assembly during cell division. This immunoassay from Assay Genie quantifies endogenous human DLGAP5 in tissue homogenates and cell lysates, supporting research in cell cycle regulation, mitosis mechanisms, and proliferation biomarker discovery. The kit is supplied in a 96 assays format with a microplate ready for standard curve establishment and unknown sample interpolation.

Elevated DLGAP5 expression has been reported in hepatocellular carcinoma, lung adenocarcinoma, triple-negative breast cancer, and high-grade glioma, where it often correlates with mitotic index, tumor grade, and shorter progression-free survival. Researchers therefore deploy this kit to monitor HURP levels in patient-derived xenografts, primary tumor biopsies, and cultured cell lines under pharmacologic perturbation, including Aurora kinase inhibitors and microtubule poisons.

Protocols typically begin with lysing cells or tissue in non-denaturing buffer supplemented with protease inhibitors, followed by serial dilution of standards to generate a calibration curve across the working range. After incubation and washing steps, the absorbance-based reaction is read on a standard plate reader, and concentrations are extrapolated against the standard curve. Reagents should be stored refrigerated upon arrival; the kit ships ready for immediate research use only.