Human DIO1/Deiodinase, Iodothyronine, Type I ELISA Kit

A colorimetric Sandwich ELISA from Assay Genie for the quantitative measurement of human DIO1 (Deiodinase Iodothyronine Type I), an enzyme involved in thyroid hormone regulation and metabolism. The kit is intended for research use and accepts serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples.

Key performance specifications reported by the manufacturer include a sensitivity of 0.188 ng/mL and an assay range of 0.313-20 ng/mL. Dilutional linearity is documented for serum (90-105%, 92-98%, 88-99%), EDTA plasma (94-101%, 85-97%, 94-100%), and heparin plasma (87-100%, 87-99%, 90-93%) at 1:2, 1:4 and 1:8 dilutions. Spike-and-recovery averages are 95% for serum, 95% for EDTA plasma and 100% for heparin plasma. The procedure uses a biotin-labelled detection antibody and HRP-Streptavidin (SABC), with TMB substrate development and absorbance read at 450 nm.