Human Dickkopf-related protein 3 (DKK3) ELISA Kit

From Assay Genie, this Sandwich ELISA kit uses a double-antibody detection method for the quantitative measurement of human Dickkopf-related protein 3 (DKK3), also known by the synonym REIC, in research samples. It is reactive with human specimens and has been validated for serum, plasma, cell culture supernatant, cell or tissue lysates, tissue homogenates, and other biological fluids. The assay covers a measurement range of 62.5–4000 pg/mL, with reagents intended to be stored at 2–8 °C for up to 12 months, and the kit is supplied for research use only.

Performance characteristics reported in the product documentation include a sensitivity of 26.5 pg/mL, intra-assay precision of CV <10%, inter-assay precision of CV <12%, and kit stability with a loss of activity of less than 5% within the expiration date under appropriate storage conditions. Linearity has been demonstrated across serial dilutions of spiked serum, EDTA plasma, and heparin plasma samples, and recovery ranges are quoted separately for each of those matrices. DKK3 is a member of the Dickkopf family known to influence the Wnt signalling pathway and to play a role in cell growth, differentiation, and apoptosis, with reported associations in research literature to cancer, cardiovascular disorders, and neurological conditions.