Human Diaphanous Homolog 1 (DIAPH1) ELISA Kit

DIAPH1 (Diaphanous Homolog 1, also called mDia1) is a member of the formin family of proteins that nucleates and elongates actin filaments downstream of Rho GTPase signaling, governing cell morphology, cytokinesis, and directed migration. Mutations in DIAPH1 cause DFNA1 non-syndromic hearing loss and have been linked to thrombocytopenia with immune dysfunction, so reliable quantification of the protein matters for cytoskeletal, auditory, and hematology research programs.

This Assay Genie ELISA kit provides all reagents needed to measure DIAPH1 in human tissue homogenates and clarified samples, supporting in vitro research applications. The immunoassay is supplied on a 96-assay microplate, leaving enough wells for a standard curve plus sample replicates. Unopened components should be stored refrigerated between uses to preserve reagent activity, and reconstituted standards can be aliquoted to limit freeze-thaw cycles.

Investigators commonly pair this ELISA with orthogonal methods such as western blot, immunohistochemistry, or flow cytometry to confirm DIAPH1 expression in cells and tissues of interest. The kit is configured for human reactivity only and is intended for research use.