Human DHCR7 (7-dehydrocholesterol reductase) ELISA KIT (HUFI05492)

The DHCR7 ELISA Kit from Assay Genie delivers quantitative measurement of 7-dehydrocholesterol reductase, the enzyme that catalyzes the final reduction step of cholesterol biosynthesis, converting 7-dehydrocholesterol to cholesterol. Investigators in cholesterol metabolism, sterol pathway research, and Smith-Lemli-Opitz syndrome (SLOS) studies use this kit to measure DHCR7 levels in human research samples.

Configured for 96 Assays, the kit uses an immunoassay workflow read on a standard microplate reader after substrate development. The reagents are supplied in vials and the assay plate is single-use, supporting batch processing of experimental and control conditions in one run. Unopened kit components should be stored refrigerated and protected from light; reconstituted standards and antibody working solutions should be kept cold between uses.

This assay supports research into SLOS, a congenital disorder caused by DHCR7 deficiency that disrupts cholesterol synthesis, as well as broader questions about hepatic and neuronal sterol metabolism. It is intended for research use only and is reactive with human serum fractions and plasma-derived specimens. Each pack contains the microplate, detection antibody, standards, and substrate buffer needed to complete the full 96-assay run.