Human DFF45/DFFA ELISA Kit (HUFI04535)

Quantify apoptosis-associated DFF45/DFFA release in human samples with this 96-test microplate immunoassay from Assay Genie. DNA Fragmentation Factor 45 (DFFA), also called DFF45, normally chaperones and inhibits caspase-activated DNase (CAD). During programmed cell death, caspase-3 cleaves DFF45/DFFA, freeing CAD to perform oligonucleosomal DNA cleavage. Measuring circulating DFF45/DFFA therefore serves as a biomarker of apoptotic activity in cancer, neurodegeneration, and autoimmune research.

The kit is validated for serum samples, plasma samples, cell culture supernatant, and tissue homogenate from human donors. The colorimetric workflow uses a pre-coated microplate, ready-to-use reagents, and a complete protocol insert. Researchers studying chemotherapeutic response, ischemic injury, or immune-mediated cell death can pair DFF45/DFFA quantification with caspase-3 activation assays and TUNEL staining for a fuller picture of programmed cell death.

Kit contents include microplate wells pre-coated with capture antibody, lyophilized protein standards and assay controls, biotinylated detection antibody, streptavidin-HRP conjugate, wash buffer, substrate solution, and stop solution. The plate-based format supports high-throughput screening of clinical cohorts and parallel in vitro experiments. Store kit components according to the supplied insert and bring all reagents to room temperature before use. For research use only.