Human Desmoglein 3 (DSG3) ELISA Kit

Quantify human Desmoglein 3 (DSG3) in tissue homogenates, cell lysates, and cell culture supernatants with this colorimetric ELISA kit from Assay Genie. DSG3 is a transmembrane cadherin component of desmosomes that mediates strong intercellular adhesion in epithelial tissues, and it is the principal autoantigen targeted in pemphigus vulgaris, an autoimmune blistering skin disorder. Researchers studying skin biology, mucosal immunity, and squamous epithelial cancers rely on DSG3 quantification to investigate disease mechanisms, biomarker expression, and antibody-mediated pathology.

Configured for 96 assays and intended for in vitro research use only, the workflow uses a colorimetric readout compatible with standard microplate readers. Reagents are supplied ready for use with the included standards and detection antibody, and the protocol follows conventional ELISA methodology that can be completed within a standard laboratory day. Each kit provides calibrated standard curves and quality-control measures to support reproducibility across runs.

Sample preparation from adherent or suspension cell cultures enables analysis of DSG3 expression under varied experimental conditions, including treatment with cytokines, pharmacologic agents, or genetic perturbations. Applications include studies on desmosome assembly, epithelial-mesenchymal transition, and tumor invasion in head and neck squamous cell carcinoma models, where DSG3 has been characterized as a biomarker of disease progression.