Human Desmoglein 1 (DSG1) ELISA Kit

Quantitative measurement of human Desmoglein 1 (DSG1) protein in tissue homogenates and cell lysates is supported by this Assay Genie ELISA Kit, which provides a chromogenic immunoassay for cell culture and tissue-based research workflows. DSG1 is a 160 kDa calcium-binding transmembrane glycoprotein component of desmosomes, the intercellular junctions that mediate keratinocyte adhesion in stratified squamous epithelia. Functional loss of desmoglein 1 is a hallmark of pemphigus foliaceus, an IgG-driven autoimmune blistering skin disease in which patient autoantibodies target the DSG1 ectodomain, and reduced DSG1 expression has additionally been reported in squamous cell carcinoma lesions of skin, esophagus, and head and neck tissues. Investigators studying desmosome assembly, epithelial barrier integrity, cadherin-mediated adhesion, or dermatological autoimmunity apply this kit to quantify endogenous DSG1 in skin biopsy homogenates, primary or differentiated keratinocyte lysates, and relevant model systems. The kit is supplied as a complete set containing a pre-coated microplate, biotinylated detection antibody, calibrated reference standards, and streptavidin-HRP conjugate for chromogenic readout. Reagents are intended for research use only and provide 96 individual assays from the supplied components.