Human DEP-1/CD148 ELISA Kit

From Assay Genie, this Sandwich ELISA kit is designed for the precise measurement of DEP-1/CD148 levels in human samples, including serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. DEP-1/CD148, also known as Density-Enhanced Phosphatase 1, is an enzyme involved in the regulation of cell signaling and growth, with roles in cell adhesion, migration, and proliferation. Dysregulation of DEP-1/CD148 has been linked to diseases including cancer, diabetes, and inflammatory disorders, making it a valuable biomarker for studying these conditions and developing potential treatments.

The kit uses a double antibody sandwich ELISA format with a reported sensitivity of 0.188 ng/mL and a measurement range of 0.313-20 ng/mL. Reported recovery averages are 96% for serum, 94% for EDTA plasma, and 95% for heparin plasma. Reported linearity across 1:2, 1:4, and 1:8 dilutions spans 85-101%, 87-100%, and 87-99% for serum; 88-104%, 88-97%, and 80-93% for EDTA plasma; and 86-103%, 82-101%, and 84-94% for heparin plasma. Colorimetric detection is performed using TMB substrate with absorbance read at 450 nm.

Stored at 2-8°C for 12 months, the kit supports research applications in cell biology.