Human Deoxyribonuclease X (DNASEX) ELISA Kit

Deoxyribonuclease X (DNASEX) is an X-chromosome-linked endonuclease that participates in apoptotic DNA fragmentation and has been investigated in spermatogenesis research and cancer biology. This absorbance-based immunoassay from Assay Genie quantifies human DNASEX protein in cleared tissue lysates and other processed biological specimens, supporting studies of nuclease regulation, DNA integrity pathways, and reproductive cell biology.

The kit ships as a complete package of pre-coated plate wells, biotinylated detection antibody, streptavidin-HRP conjugate, calibrated recombinant standards, wash buffer concentrate, and substrate solution, providing enough reagents for the full plate run. Researchers load prepared samples alongside the standard curve, incubate, develop colour, and read absorbance to interpolate DNASEX concentrations across their dilution series. Each kit supports in vitro research use only and should be stored refrigerated at 4 °C between uses to preserve antibody and conjugate activity.

Reconstituted standards and working detection antibody remain stable through short-term use when kept cold, and the kit is supplied in a sealed foil pouch with desiccant to protect strip wells from moisture. The strip-plate format of eight removable strips of twelve wells accommodates both small pilot studies and larger sample cohorts, and laboratories frequently pair DNASEX measurements with downstream DNA fragmentation assays and western blot validation. Sample handling guidance, reconstitution volumes, and dilution recommendations are detailed in the lot-specific manual enclosed in the box.