Human Deoxyribonuclease gamma (DNASE1L3) ELISA Kit

Manufactured by Assay Genie, this Sandwich ELISA Kit is designed for the quantitative measurement of human Deoxyribonuclease gamma (DNASE1L3) in biological samples. The kit uses a colorimetric detection method and reacts with human samples, making it suitable for research into DNase1L3 — a key enzyme involved in DNA degradation, genomic integrity maintenance, and immune regulation. Dysregulation of DNase1L3 has been implicated in autoimmune diseases, inflammatory conditions, and certain cancers, underscoring its value as a biomarker for studying these disease states and potential therapeutic targets.

The assay delivers a detection range of 0.312–20 ng/mL with a sensitivity of 0.18 ng/mL. Precision is reported as an intra-assay CV of 8.9% and an inter-assay CV of 11.5%. Linearity has been validated across serial dilutions (1:2, 1:4, 1:8, 1:16) in serum (93–115%), EDTA plasma (83–110%), and heparin plasma (80–118%), with serum recovery averaging 94% (range 88–100%) and plasma recovery averaging 96% (range 90–102%). The kit is specific for natural and recombinant human Deoxyribonuclease gamma and is intended for research use only.

Acceptable sample types include serum, plasma, tissue homogenates, cell culture supernatants, and other biological fluids, supporting applications across epigenetics research and studies of DNA fragmentation during apoptosis. Synonyms annotated for the target include DNase I homolog protein DHP2, Deoxyribonuclease I-like 3, Liver and spleen DNase, LS-DNase, and DNase gamma (UniProt: Q13609).