Human Defensin Alpha 6, Paneth Cell Specific (DEFa6) ELISA Kit

Paneth cells anchored at the base of the small intestinal crypts secrete Defensin Alpha 6, a small antimicrobial peptide that contributes to innate mucosal defense against enteric bacteria and helps sculpt the composition of the resident gut microbiome. Researchers studying mucosal immunology, inflammatory bowel disease, Crohn's disease, and Paneth cell dysfunction use this immunoassay kit from Assay Genie to quantify DEFA6 levels across biological specimens with target specificity.

The assay is configured for 96 individual determinations and is applicable to serum samples, plasma preparations, cell lysates, and homogenized tissue specimens. Each kit is supplied as a complete reagent set containing a pre-coated microplate, recombinant DEFA6 standard, biotinylated detection reagent, streptavidin-enzyme conjugate, wash concentrate, dilution buffers, chromogenic substrate, stop reagent, and adhesive plate sealers. All components are shipped on cold packs and should be stored refrigerated upon arrival, with reconstituted reagents kept frozen for longer-term use to preserve activity through repeated freeze-thaw cycles.

Common applications include measuring secreted DEFA6 levels in conditioned media from intestinal organoid cultures, primary Paneth cell preparations, and biopsy-derived tissue samples, as well as probing correlations between defensin expression and disease activity in inflammatory bowel disorder cohorts.