Human Defensin Alpha 3, Neutrophil Specific (DEFa3) ELISA Kit

The Assay Genie DEFa3 immunoassay kit provides quantitative measurement of Human Defensin Alpha 3, also designated HNP-3 (Human Neutrophil Peptide 3), a cationic antimicrobial peptide constituent of the azurophilic granule repertoire deployed by polymorphonuclear neutrophils during innate immune responses. The kit supports research into antimicrobial defense mechanisms, with established relevance to studies of bacterial and fungal infection dynamics, sepsis biomarker investigation, chronic inflammatory conditions including inflammatory bowel disease, and mucosal immunity research. It is configured as a complete reagent set intended for in vitro research use only and is supplied as a kit of ninety-six individual determinations with reagents presented in vials. Compatible sample types for this kit include serum specimens, plasma specimens, tissue homogenate preparations, cell lysate extracts, and cell culture supernatant fractions.

Defensin Alpha 3 belongs to the alpha-defensin subfamily and is co-expressed with HNP-1, HNP-2, and HNP-4 in human neutrophils, although HNP-3 is typically present at substantially lower abundance than the dominant HNP-1 and HNP-2 species. Researchers investigating neutrophil degranulation, host-pathogen interaction, or biomarker discovery in respiratory and gastrointestinal inflammation contexts commonly reference this target. The absorbance-based readout method relies on standard laboratory microplate instrumentation. Reagents should be handled according to the enclosed package insert, and the kit is intended for research use only, not for diagnostic or clinical procedures.