Human Defensin-5 (DEFA5) ELISA Kit

Manufactured by Assay Genie, this sandwich ELISA kit is designed for the quantitative detection of human Defensin-5 (DEFA5) in serum, plasma, tissue homogenates, cell culture supernates and other biological fluids. The assay uses a colorimetric detection method and is configured for use with human samples, making it suitable for research into antimicrobial peptides and immune-related conditions.

Performance characteristics include a detection range of 15.6-1000pg/mL and a sensitivity of 8.9pg/mL, with intra-assay CV of 8.1% and inter-assay CV of 9.2%. Linearity has been validated in serum, EDTA plasma, and heparin plasma across dilution factors of 1:2, 1:4, 1:8, and 1:16, while recovery is reported as 100% (range 94-106%) for serum and 102% (range 96-108%) for plasma. The kit specifically detects natural and recombinant human Defensin-5, a homodimeric antimicrobial peptide with a molecular weight of 10,071 Da that exerts antimicrobial activity against Gram-negative and Gram-positive bacteria.

Provided in a solid phase format for research use only. Kits are shipped at +4°C, with components stored at -20°C or 4°C as detailed in the included protocol.