Human DEFB4A/Beta-defensin 4A ELISA Kit

Assay Genie provides a Sandwich ELISA Kit for the quantitative measurement of human DEFB4A, also known as Beta-defensin 4A. Built on a double-antibody sandwich format, the assay offers high sensitivity and specificity for precise and reproducible results. DEFB4A is a key component of the innate immune system that plays a crucial role in defending against microbial pathogens, with its expression linked to various inflammatory conditions and diseases.

The kit delivers a sensitivity of 37.5 pg/mL and a standard range of 62.5-4000 pg/mL. It accepts a broad variety of human sample types, including serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. Detection is colorimetric, with absorbance measured at 450 nm following TMB substrate development and a stop reaction. Kit components are stored at 2-8°C for 12 months. Linearity has been validated in serum, EDTA plasma, and heparin plasma across 1:2, 1:4, and 1:8 dilutions, with recovery rates averaging 92% in serum, 91% in EDTA plasma, and 96% in heparin plasma.

Relevant to cell biology and immunology research, the kit enables accurate quantification of DEFB4A in biological samples and supports investigation of immune responses, infection, and inflammatory disorders, providing a basis for new insights into host defense and potential therapeutic interventions.