Human DEFB127/ (Beta-defensin 127) ELISA Kit

Manufactured by Assay Genie, this Sandwich ELISA kit is designed for the quantification of human DEFB127 (Beta-defensin 127), an antimicrobial peptide involved in the innate immune response. The double-antibody sandwich format is validated for use with human serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples, with a stated sensitivity of 56.25 pg/mL and a detection range of 93.75-6000 pg/mL. The kit is read colorimetrically at 450 nm, and components should be stored at 2-8°C for up to 12 months.

Manufacturer validation data report linearity values between 82% and 104% across serum, EDTA plasma, and heparin plasma at dilutions from 1:2 to 1:8. Recovery ranges from 86% to 105%, with matrix-specific averages of 95% (serum), 98% (EDTA plasma), and 92% (heparin plasma). The assay workflow comprises primary incubation at 37°C, biotinylated detection antibody binding, HRP-streptavidin (SABC) binding, and TMB substrate development. DEFB127 is of research interest as a potential biomarker for immune function, infectious disease, and inflammatory conditions.