Human DEFB-3/Beta-defensin 103/BD-3 PharmaGenie ELISA Kit (SBRS0494)

A solid-phase immunoassay from Assay Genie quantifies human beta-defensin 103 (BD-3, DEFB-3), a small cationic antimicrobial peptide expressed by epithelial cells at mucosal and skin surfaces. The peptide contributes to innate immunity through membrane-disrupting activity against Gram-positive bacteria, Gram-negative bacteria, and certain fungi, making it a target of interest in dermatology, mucosal biology, inflammation research, wound healing studies, and host-pathogen interaction work.

The kit is supplied as 96 assays and is configured for measuring the analyte in cell culture supernatants and in circulating blood-derived specimens such as plasma or serum. Each run uses a precoated microplate and produces a colorimetric endpoint that is read on a standard plate reader.

All reagents are intended for research use only and should be kept refrigerated; protect components from light exposure during storage and handling. The assay supports in vitro workflows including epithelial cell culture studies, biomarker screening of clinical samples, and experimental infection or skin barrier models. Standard immunoassay practice applies: avoid repeated freeze-thaw cycles of prepared standards, and use freshly aliquoted material where possible to preserve the analyte.

The package contains the precoated plate, detection antibody, recombinant standards, buffers, and substrate sufficient to perform 96 individual determinations, supporting both small pilot studies and routine screening campaigns in academic and industrial laboratories.