Human DDOST ELISA Kit

From Assay Genie, this sandwich ELISA kit employs a double-antibody format for the quantitative detection of human DDOST. The assay is compatible with serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid sample types, and uses a colorimetric read-out with absorbance measured at 450 nm. Reported sensitivity is 0.188 ng/mL and the standard range is 0.313-20 ng/mL. Synonyms annotated for the target include DDOST, OK, SW-cl.45, KIAA0115, OST48, DDOST 48 kDa subunit, and Oligosaccharyl transferase 48 kDa subunit (UniProt P39656); the kit is positioned within the cell biology research area.

The protocol comprises reagent and plate preparation, a 90-minute primary incubation at 37°C, a 60-minute biotin-labelled detection antibody step, a 30-minute HRP-Streptavidin (SABC) binding step, TMB substrate development in the dark for 10–20 minutes, and a stop reaction read at 450 nm. Reagents are stored at 2-8°C for 12 months. Sample-preparation guidance covers serum (clot, 1000 × g, 20 minutes), plasma (anticoagulant tubes, 1000 × g, 15 minutes at 2-8°C), tissue homogenate, cell culture supernatant (2500 rpm, 5 minutes), and cell lysate workflows.

Validation data include linearity for serum at 1:2/1:4/1:8 of 87-101%, 87-98% and 97-104%; EDTA plasma 84-100%, 86-101% and 85-101%; and heparin plasma 80-92%, 86-98% and 85-94%. Recovery ranges average 96% in serum (90-102%), 95% in EDTA plasma (91-103%) and 95% in heparin plasma (87-104%).