Human DDO/D-aspartate oxidase ELISA Kit

From Assay Genie, this Sandwich ELISA, Double Antibody kit is designed for the quantitative measurement of human DDO/D-aspartate oxidase in serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. The assay is read colorimetrically at 450 nm after TMB color development, and the kit is shipped for storage at 2-8°C (with select components suitable for -20°C long-term storage as listed in the manual).

Performance characteristics reported by the manufacturer include a sensitivity of 0.188 ng/mL and an assay range of 0.313-20 ng/mL. Linearity across 1:2, 1:4, and 1:8 dilutions (n = 5) is reported as 96-105% / 87-101% / 85-101% for serum, 82-97% / 82-98% / 86-99% for EDTA plasma, and 82-99% / 81-100% / 85-95% for heparin plasma. Spike-and-recovery performance is given as ranges with average percentages: serum 90-101% (average 95%), EDTA plasma 87-100% (average 94%), and heparin plasma 85-103% (average 94%).

The literature describes DDO as an enzyme involved in D-aspartate metabolism, with abnormal levels implicated in neurological disorders, metabolic diseases, and kidney dysfunction. The kit is therefore positioned for research use in cell biology and related investigations of DDO's physiological and pathological roles.