Human D-beta-hydroxybutyrate dehydrogenase, mitochondrial (BDH1) ELISA Kit

From Assay Genie, this sandwich ELISA kit is designed for the precise quantification of human D-beta-hydroxybutyrate dehydrogenase, mitochondrial (BDH1), in serum, plasma, tissue homogenates, cell culture supernatants and other biological fluids. The target is a key mitochondrial enzyme involved in ketone body metabolism that plays a crucial role in energy production during fasting or low carbohydrate intake, and abnormal levels have been linked to metabolic disorders such as diabetes and obesity. The kit is intended for research use only.

Manufacturer-quoted performance characteristics include a detection range of 31.2–2000 pg/mL, a sensitivity of 10.2 pg/mL, an intra-assay CV of 5.1%, and an inter-assay CV of 7.8%. Recovery is reported as 100% (range 94–106%) in serum and 102% (range 96–108%) in plasma. The assay is specific for natural and recombinant human D-beta-hydroxybutyrate dehydrogenase (mitochondrial), with the target protein described as a homotetramer localised to the mitochondrial inner membrane and matrix (UniProt Q02338). The listed research area is cardiovascular, and synonyms for the target include 3-hydroxybutyrate dehydrogenase, short chain dehydrogenase/reductase family 9C member 1, BDH, and SDR9C1.