Human D-Amino Acid Oxidase (DAO) ELISA Kit

Assay Genie's quantitative immunoassay for D-Amino Acid Oxidase supports research into peroxisomal flavin enzyme activity in human tissue. DAO oxidizes neutral D-amino acids, particularly D-serine and D-alanine, and regulates NMDA receptor co-agonist availability in the central nervous system. Investigators studying schizophrenia, amyotrophic lateral sclerosis, and other neuropathologies use this ELISA to quantify DAO protein in tissue homogenates for in vitro research, with the 96-assay plate format providing sufficient wells for standard curves and replicate samples.

The kit ships in a refrigerated pack containing antibody-coated wells, lyophilized standards, biotinylated detection antibody, streptavidin-HRP conjugate, wash buffer concentrate, substrate solution, and stop reagent. Researchers run the assay at room temperature and read absorbance after adding the stop reagent. Each kit includes a complete protocol insert with recommended dilutions for brain, kidney, and liver homogenates, the tissues where DAO expression is highest. Store unopened components at 4 degrees C and protect the substrate from light until use.