Human Cytoplasmic Antiproteinase 3 (CAP3) ELISA Kit

CAP3, also known as azurocidin or heparin-binding protein, is a neutrophil granule-derived serine protease homolog implicated in sepsis prognosis, acute inflammation, and neutrophil activation studies. Researchers using this Assay Genie kit can pair quantitative CAP3 readouts with parallel myeloperoxidase or elastase measurements to profile the azurophilic granule release cascade in patient cohorts.

The calibrator series is prepared against a recombinant CAP3 reference, supporting inter-batch reproducibility across longitudinal studies. Where elevated CAP3 concentrations are expected, matched sample matrices are commonly diluted 1:2 to 1:10 in the supplied assay diluent, while tissue homogenates may require higher dilution factors depending on organ source. Internal validation reports typical spiking recovery within 80-120% across the tested matrices, with intra-assay and inter-assay CVs generally below 10%.

Reagent volumes are sized for a full plate run with reserve, allowing researchers to process partial plates without exhausting the biotinylated detector or streptavidin-HRP conjugate. Unopened components should be refrigerated on arrival, and the reconstituted standard along with the biotin conjugate should be aliquoted and stored at -20 degrees C to limit freeze-thaw cycles and preserve antigen binding activity.