Human Cytokine Receptor Like Factor 1 (CRLF1) ELISA Kit

Quantitative immunoassay from Assay Genie for measuring Cytokine Receptor Like Factor 1 (CRLF1) in human research samples. CRLF1 is a soluble cytokine-binding partner that heterodimerizes with cardiotrophin-like cytokine factor 1 (CLCF1) to form the CLC complex, which activates gp130/JAK/STAT signaling and supports motor neuron survival, glial differentiation, and thermoregulatory neural circuit function. Published studies link CRLF1 sequence variants to cold-induced sweating syndrome (CISS) phenotypes, and altered CRLF1 expression has been investigated in hematopoietic and inflammatory research contexts.

The kit is supplied as a complete package containing a microplate pre-coated with capture antibody, biotinylated detection reagent, streptavidin-HRP conjugate, standard calibrator, and assay buffer sufficient for 96 individual assays on a single plate. Researchers apply it to cell culture supernatant analysis, tissue homogenate studies, serum-based biomarker work, and investigations of CRLF1 pathway involvement in neuromuscular and immune signaling.

Each pack of 96 assays supports standard curve generation plus multiple sample replicates. Reagents should be kept refrigerated upon receipt to preserve binding activity through the kit shelf life. The protocol follows a standard ELISA workflow with wash, incubation, and chromogenic development steps, with absorbance readings taken on a standard microplate reader.