Human Cytochrome P450 7A1 (CYP7A1) ELISA Kit

CYP7A1, also known as cholesterol 7α-hydroxylase, is the rate-limiting hepatic enzyme that converts cholesterol into 7α-hydroxycholesterol, the first committed step of the classical bile acid synthesis pathway. This Assay Genie kit supports quantitative measurement of human CYP7A1 protein in tissue homogenates and cell lysates, enabling researchers to monitor how the enzyme responds to dietary cholesterol intake, bile acid feedback through FXR signaling, and pharmacological modulation of lipid metabolism.

The product is configured as a 96 assays format ELISA with a colorimetric endpoint, read on standard microplate readers, with each well receiving a small-volume sample during the run. Reagents supplied include pre-coated plate strips, capture antibody, biotinylated detection antibody, recombinant standard, wash buffer, substrate, and stop solution sufficient to complete the full protocol.

Storage guidance includes keeping standards and detection antibody refrigerated and protected from light until use, while substrate and stop solutions are typically reserved for short-term bench use. Applications span studies of bile acid homeostasis, non-alcoholic fatty liver disease progression, dyslipidemia, and cholesterol catabolism, with the assay designated for research use only and not for clinical diagnostic procedures.