Human Cytochrome P450 27B1 (CYP27B1) ELISA Kit

The Human Cytochrome P450 27B1 (CYP27B1) ELISA Kit from Assay Genie is a quantitative immunoassay configured on a 96-well microplate for measuring CYP27B1 protein in human specimens. CYP27B1 encodes the mitochondrial enzyme 1α-hydroxylase, which catalyzes the conversion of 25-hydroxyvitamin D3 into the active hormone 1,25-dihydroxyvitamin D3 (calcitriol), making this assay a useful tool for vitamin D metabolism, calcium homeostasis, and bone mineralization research.

Each kit supplies a pre-coated microplate, biotinylated detection antibody, lyophilized protein standards, and streptavidin-HRP conjugate. The protocol requires a 100 uL sample volume per well, and the capture antibody is human-specific with no significant cross-reactivity observed against recombinant CYP27A1, CYP24A1, or CYP2R1. Reconstituted standards should be aliquoted and frozen at -20°C to limit freeze-thaw cycles, while unopened kit components are refrigerated and stored at 4°C. Intended for research use only; in vitro diagnostic applications are not supported.