Human Cytochrome P450 27A1 (CYP27A1) ELISA Kit

Mitochondrial sterol 27-hydroxylase (CYP27A1) initiates the acidic pathway of bile acid biosynthesis by oxidising cholesterol and related sterol intermediates at the C27 position. Dysregulation of this cytochrome P450 enzyme is implicated in cerebrotendinous xanthomatosis, hepatic sterol homeostasis, vitamin D metabolism, and macrophage lipid turnover research.

This Assay Genie ELISA Kit supports quantitative measurement of human CYP27A1 protein in tissue homogenates, cell lysates, and related experimental sample matrices. The colorimetric assay is provided in a 96-assay microplate format intended for batched research workflows and applies to in vitro studies of cholesterol handling, drug metabolism investigations, and biomarker discovery work. Endogenous enzyme levels can be tracked across cell culture models and animal tissue samples to characterise pathway activity under different physiological or pharmacological conditions.

For research use only, the kit complements western blot, PCR, and immunohistochemistry workflows that profile P450 family expression in hepatic, neural, and peripheral tissue contexts.