Human Cytochrome C Oxidase Subunit IV Isoform 1 (COX4I1) ELISA Kit

Researchers studying mitochondrial biology and respiratory chain function can use this Assay Genie kit to quantify Cytochrome C Oxidase Subunit IV Isoform 1 (COX4I1), a nuclear-encoded component of complex IV in the electron transport chain. The assay is configured for use with human cell lysates and tissue homogenates, making it appropriate for cell biology, metabolism, and neurodegeneration research where mitochondrial mass is being characterized.

COX4I1 is widely employed as a mitochondrial loading marker alongside proteins such as VDAC1 and TOM20, and changes in its measured abundance reflect altered mitochondrial content in cultured cells and primary tissues. Reagents are supplied in a microplate-based format, and the kit is intended for research use only in laboratory settings where standardized ELISA workflows are already in place. Components should be stored according to the package insert, which protects antibody and conjugate stability across the kit shelf life; reconstituted materials are commonly aliquoted to limit freeze-thaw cycles before each experiment.

Common applications include validating mitochondrial enrichment prior to western blot analysis, comparing COX4I1 levels across patient cohorts, and tracking respiratory chain subunit expression after pharmacological or genetic perturbation in vitro. Cell lysates prepared with RIPA buffer and freshly snap-frozen tissue homogenates serve as compatible inputs for the workflow.