Human Cytochrome c oxidase subunit 3 (MT-CO3) ELISA Kit

Assay Genie supplies a Sandwich ELISA Kit for the quantitative detection of Human Cytochrome c oxidase subunit 3 (MT-CO3) in human samples, with reactivity limited to human specimens. The kit is applicable to serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids, and uses a colorimetric detection method. Specificity covers natural and recombinant human MT-CO3. MT-CO3 is a subunit of cytochrome c oxidase, the terminal enzyme of the mitochondrial electron transport chain that generates ATP, and its dysregulation has been linked to mitochondrial disorders, neurodegenerative diseases, and cancer.

Reported performance figures include a detection range of 0.156-10ng/mL, a sensitivity of 0.1ng/mL, an intra-assay CV of 5.1%, and an inter-assay CV of 9.6%. Linearity values (N=5) span 105-117% to 105-115% for serum, 109-119% to 104-116% for EDTA plasma, and 111-120% to 105-105% for heparin plasma across dilutions from 1:2 to 1:16. Recovery averages 106% (range 100-112%) in serum and 108% (range 102-114%) in plasma. The target is a multi-pass inner mitochondrial membrane protein (UniProt P00414), and the kit is designated for research use only within the neurosciences area.