Human Cytochrome b-245 Beta Polypeptide ELISA Kit (HUFI08038)

The Human Cytochrome b-245 Beta Polypeptide ELISA Kit from Assay Genie targets CYBB (gp91phox), the catalytic membrane subunit of the phagocyte NADPH oxidase complex. Researchers apply this assay to investigate chronic granulomatous disease (CGD), evaluate neutrophil respiratory burst capacity, and explore innate immune signaling pathways.

The kit contains a pre-coated microplate, biotinylated detection antibody, streptavidin-HRP conjugate, calibrated reference standards, dilution buffer, wash buffer concentrate, TMB substrate, and stop solution, all supplied in single use vials. The protocol calls for a standard 50 ul sample per well with sequential incubation and wash steps. Antibody aliquots and standards should be kept frozen and protected from repeated freeze-thaw cycles to maintain activity over the kit shelf life.

This assay is commonly cited in studies of X-linked immunodeficiency, oxidative burst in macrophages and neutrophils, and biomarker discovery projects that connect NADPH oxidase dysfunction to inflammatory and autoimmune phenotypes. The kit is calibrated against recombinant human CYBB reference material and validated for research use only in qualified laboratory settings.