Human Cystatin-B (CSTB) ELISA Kit

Manufactured by Assay Genie, this sandwich-format ELISA is designed for the quantitative detection of cystatin B in human samples, including serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids. It uses a colorimetric ELISA detection method and is reactive with natural and recombinant human Cystatin-B (also known by synonyms including CPI-B, Liver thiol proteinase inhibitor, Stefin-B, CST6, and STFB), a cytoplasmic and nuclear intracellular thiol proteinase inhibitor that functions as a tightly binding reversible inhibitor of cathepsins L, H and B.

According to the manufacturer's specifications, the kit has a detection range of 1.56-100ng/mL and a sensitivity of 0.88ng/mL. Reported precision values are an intra-assay CV of 5.1% and an inter-assay CV of 8.2%. Linearity has been validated across serum, EDTA plasma, and heparin plasma at dilutions of 1:2, 1:4, 1:8, and 1:16 (N=5). Recovery averages 103% (range 97-109%) for serum and 105% (range 99-111%) for plasma. The kit is intended for research use only and is associated with the research area of epigenetics, with the target protein referenced under UniProt P04080.