Human CYP3A4/Cytochrome P450 3A4 ELISA Kit

Assay Genie offers a sandwich ELISA, double antibody-based kit for the quantitative measurement of human CYP3A4 (Cytochrome P450 3A4) in serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. The kit uses a colorimetric detection method with TMB substrate and absorbance read at 450 nm.

Performance characteristics include a sensitivity of 46.875 pg/mL and an assay range of 78.125–5000 pg/mL. Linearity for serum, EDTA plasma, and heparin plasma falls within 80–105% across 1:2, 1:4, and 1:8 dilutions, while recovery ranges from 86–105% with averages of 93–99%. CYP3A4 is a key hepatic enzyme involved in drug metabolism, and the kit supports research into drug interactions, pharmacogenomics, and personalized medicine.

The assay procedure involves reagent equilibration, primary incubation at 37°C for 90 minutes, biotin-labeled detection antibody binding, HRP-streptavidin incubation, TMB color development in the dark, and stop solution addition. Provided components include a pre-coated dismountable microplate, lyophilized standard, biotin-labeled antibody, HRP-streptavidin conjugate, TMB substrate, sample and antibody dilution buffers, SABC dilution buffer, stop solution, wash buffer, plate sealer, and a technical manual. Uniprot reference: P08684. Storage is at 2-8°C for 12 months.