Human CYP2B6/Cytochrome P450 2B6 protein ELISA Kit

A high-quality, sensitive Sandwich ELISA kit from Assay Genie designed for the accurate measurement of human CYP2B6 (Cytochrome P450 2B6) protein. The assay is configured as a double-antibody sandwich ELISA and is intended for use with serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. Storage is recommended at 2–8°C for 12 months.

According to Assay Genie, the kit shows a detection sensitivity of 0.188 ng/mL and an assay range of 0.313–20 ng/mL. Linearity data (n = 5) are reported across 1:2, 1:4, and 1:8 dilutions, with serum ranging 85–104%, EDTA plasma ranging 83–100%, and heparin plasma ranging 81–99%. Recovery averages are reported as 97% across serum, EDTA plasma, and heparin plasma (n = 5), with recovery ranges of 85–105% (serum), 91–105% (EDTA plasma), and 87–105% (heparin plasma).

The protocol proceeds through reagent and plate preparation, a primary incubation at 37°C for 90 minutes, biotin-labelled detection antibody incubation at 37°C for 60 minutes, HRP-Streptavidin (SABC) binding at 37°C for 30 minutes, TMB colour development in the dark for 10–20 minutes, and a stop reaction with absorbance measured at 450 nm. CYP2B6 is highlighted as a key enzyme in drug metabolism, relevant to pharmacogenomic studies, drug-drug interactions, and personalised medicine research.